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diff --git a/testing/web-platform/tests/shadow-dom/focus/focus-tabindex-order-shadow-zero-host-negative.html b/testing/web-platform/tests/shadow-dom/focus/focus-tabindex-order-shadow-zero-host-negative.html
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+++ b/testing/web-platform/tests/shadow-dom/focus/focus-tabindex-order-shadow-zero-host-negative.html
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+<!DOCTYPE html>
+<meta charset="utf-8">
+<title>HTML Test: focus - the sequential focus navigation order with shadow dom and negative host tabindex</title>
+<link rel="help" href="https://html.spec.whatwg.org/multipage/interaction.html#sequential-focus-navigation">
+<script src="/resources/testharness.js"></script>
+<script src="/resources/testharnessreport.js"></script>
+<script src="/resources/testdriver.js"></script>
+<script src="/resources/testdriver-vendor.js"></script>
+<script src="resources/shadow-utils.js"></script>
+<body>
+<script>
+// Structure:
+// <div #aboveHost tabindex=0>
+// <div #host tabindex=-1>
+//    #shadowRoot
+//      <div #aboveSlot tabindex=0>
+//      <slot #slotAbove tabindex=0>
+//        (slotted) <div #slottedAbove tabindex=0>
+//      <slot #slotBelow tabindex=0>
+//        (slotted) <div #slottedBelow tabindex=0>
+//      <div #belowSlot tabindex=0>
+// <div #belowHost tabindex=0>
+
+promise_test(() => {
+  let elementsInFlatTreeOrder;
+  let [aboveHost, host, aboveSlot, slotAbove, slottedAbove, slotBelow, slottedBelow, belowSlot, belowHost] =
+    elementsInFlatTreeOrder = prepareDOM(document.body, false);
+  setTabIndex(elementsInFlatTreeOrder, 0);
+  host.tabIndex = -1;
+  resetFocus();
+  // Focus willl only move within the focus navigation scope of the document (not going to get into #host).
+  return assertFocusOrder([aboveHost, belowHost]);
+}, "Order when all tabindex=0 except for host, which has tabindex=-1");
+</script>
+</body>